Identification of Trichothecium roseum developing secondarily to gray rot caused by Botrytis cinerea isolated from grapes in Turkey

Aims: Identification of Trichothecium roseum infecting grapes first time in Turkey.Methods and Results: During the summer 2020, white pale pink stains were observed on vineyards harvested storages. These resembling powdery mildew clusters, mostly developing secondarily to gray rot caused by Botrytis cinerea. To determine causal agent disease symptoms, some symptomatic clusters collected both from storages located Hassa district Hatay province Turkey. Two different methods used isolate fungus tissues. For molecular identification, ITS rDNA gene sequence amplified using ITS1 ITS4 primers. Spore suspension was sprayed detached vine organs such as leaf, bud, stem cluster, after 7 days diseases symptoms those orchard. ITS1/4 primer pairs produced about 700 bp band agarose gel. Based morphologic characteristics, identified it has been for Turkey with this study.Conclusions: is present there no difference incidence or prevalence cultivation areas Turkey.Significance Impact Study: detected study. This does not cause significant economic damage directly, but causes health problems due mycotoxins which are fungus.